骨髓中的內皮細胞和血管周圍細胞是負責造血幹細胞的維持功能.Study demonstrates Endothelial and perivascular cells are responsible for Blood #stemcell maintenance

造血幹細胞壁龕研究: 小鼠體內實驗: 運用選擇性敲入綠色熒光的模仿基因, 成功演示骨髓中的內皮細胞( Endothelial cells)和血管周圍細胞 (perivascular cells)是負責 造血幹細胞 (HSC)的維持功能.


Conditional knock-in of a loss-of-function SCF-GFP gene demonstrates Endothelial and perivascular cells are responsible for HSC maintenance.


http://www.nature.com/nature/journal/v481/n7382/full/nature10783.html


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Nature. 2012 Jan 25;481(7382):457-62. doi: 10.1038/nature10783.

Endothelial and perivascular cells maintain haematopoietic stem cells.

Ding L, Saunders TL, Enikolopov G, Morrison SJ.

Source

Howard Hughes Medical Institute, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.

Abstract

Several cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenancefactors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem cell factor (SCF; also known as KITL) is a key niche component that maintains HSCs. Here, using Scf(gfp) knock-in mice, we found that Scf was primarily expressed by perivascular cells throughout the bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, nestin-cre- or nestin-creER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. Thus, HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance.

Comment in

• Nature. 2012;481(7382):453-5.